Western Blot signal booster

A short protocal version

membrane → booster → blocking → antibody(sample) → ECL

Important notes:

1. Signal enhancement is better for NC than PVDF membrane.
2. Signal enhancement is better for PVDF that is stored in water or PBS.Dried PVDF membrane is more resistant to western blot booster.

Procedure for Western blot booster

1. Wash the membrane (NC or PVDF) with deionized water three times, each for few seconds.
2. Cover the membrane with solution A and allow for incubation for 5 min at room temperature.
3. Wash the membrane with deionized water three times, each for few seconds.
4. Cover the membrane with solution B and allow for incubation for 5 min at room temperature.
5. Proceed with blocking the membrane with skim milk or other appropriate blockers.This treatment of membrane would lead to enhanced signal intensity by several folds.

Storage and Stability:

Stable when stored at room temperature. Use sterile condition for removing solution from the container, when used properly it is good for one year to use.