因此,為了適應目前廣泛的研究,「Labo Banker」是一種具有高冷凍保護性能、同時將DMSO濃度保持在10%的保存液。
即使是對冷凍敏感的細胞也可以放心地冷凍保存在冰箱中。
此外,與血清型凍存液相比,無血清凍存液的冷凍保護性能通常較差,這限制了可用的細胞類型。由於Labo Banker的無血清型具有與血清型相同的高性能,而擴大了使用無血清冷凍保存液的可能性。
品名 | 規格 | 適用 | 包裝 |
Labobanker 1 | 含血清、10% dmso | 一般細胞 | 100ml |
Labobanker 2 | 不含血清、不含動物蛋白 | 對血清敏感之細胞株 幹細胞 細菌及 病毒 (E.coli , Baculovirus) | 100ml |
labobanker使用說明
1. Remove the medium from the culture dish and wash the cells by PBS.
2. After removing PBS from the culture dish, add Trypsin/EDTA-PBS and incubate the cells for 5min at RT.
3. Add the new medium with FBS to the culture dish and gently isolate the cells from the culture dish by pipetting.
4. Transfer the cell suspension to centrifuge tube and count the cell numbers.
5. Centrifuge cell suspension at approximately 200 to 300 x g for 5 minutes and remove the supernatant.
6. Resuspend the pellet in cold (2 to 8 *C) Labobanker at 5 x 10s to 5 x 10s cells/ml.
7. Divide this cell suspension into cryotubes at 0.5 to 1.0 ml/tube.
8. Leave the cell in the cryotubes on ice for 5 minutes.
9. Directly freeze the cell in a deep freezer at -80 C.
10. In case of long-term presevation, transfer the frozen cells at -80*C into a liquid nitrogen (-196 *C) on the
following day.