DESCRIPTION:
The EasyPure™ Gel/PCR DNA Fragments Extraction Kit is designed to recover or concentrate
DNA fragments (50bp-10Kb) from agarose gels, PCR or other enzymatic reactions. The unique
dual purpose application and high yield DNA/minicolumn make this kit an exceptional value.
The method uses a chaotropic salt, guanidine thiocyanante to dissolve agarose gel and
denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of
the spin column. Following washing off of contaminants, the purified DNA fragments are eluted
by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides
are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation.
PG100
PG300
PG Column/collection tube
