Description:
BioGreen™ Safe DNA Gel bu er is an innovated nucleic acid stain to replace highly toxic ethidium bromide (EtBr) for detecting nucleic acid agarose gels.
It emits green uorescence when bound to DNA .
This new stain has two uorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm.
In addition, it has one visible excitation at 491 nm .
The Fluorescence emission of BioGreen™ Safe DNA Gel bu er bound to DNA is centered at 530 nm.
BioGreen™ Safe DNA Gel bu er is as sensitive as EtBr and the staining protocol is also very similar, however, compared to EtBr, which is known as a strong mutagen, BioGreen™ Safe DNA Gel bu er causes much fewer mutations in the Ames test.
In addition, it has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.
Most importantly, it is not considered hazardous waste, can be disposed of according to standard laboratories procedures.