Kits MagCore 300x228

110, MagCore® Cultured Cells DNA Kit

MagCore® Cultured Cells DNA Kit is designed to extract genomic DNA from up to 5×10⁶ cultured cells by performing with the MagCore instrument. The kit contains all required reagents and labware for automated extraction using magnetic-particle technology. Reagents necessary for a complete process are supplied in prefilled cartridges, which can be easily loaded into the MagCore® instrument.

分類: 設備 Instruments, 全自動核酸提取儀, MagCore® 系列核酸萃取試劑盒

商品介紹

110

For extraction of genomic DNA from cultured cells and amniotic fluid.
Applicable models : HF16, Compact, HF48, Super, HF16 Plus, Plus II

For 200 µl sample volumes (up to 5 x10⁶ cells).

Contents : pre-filled cartridges, proteinase K, PK storage buffer, disposable tip & holder sets, sample tubes, elution tubes
Shelf life : 18 months

Features

High performance of purified DNA in downstream applications such as qPCR.
Reliable isolation of DNA up to 5 x106 cells
Pre-filled and sealed buffer cartridges prevent contamination.
No phenol or chloroform extraction.
Efficient removal of contaminants and inhibitors.

Applications

The high quality DNA obtained is suited for many applications, such as:

PCR and real-time PCR
Next Generation Sequencing (NGS)

DNA quality analysis by Spectrophotometer

DNA of three different cultured cells were extracted by the employment of MagCore® HF16 and MagCore® Cultured Cells DNA Kit (200μl sample volume).

The following data show the yield and purity of DNA of three samples.

1	2	3
Sample	COS 7 (1.2×10⁷)	Junkat (1×10⁶)	HEK293 (1×10⁶)
Yield (mg)	16.44	13.08	24.42
DNA purity (A₂₆₀/₂₈₀)	1.93	1.87	1.92

Amniotic fluid DNA extration

Result

DNA quality is checked by the NanoDrop spectrophotometer and agarose gel electrophoresis after DNA purification from amniotic-fluid cells. Cells were harvested from 10~15 ml amniotic fluid samples at pregnancy weeks 16-18 by centrifugation for 10 minutes at 3000 rpm.

Sample ID		Conc. (pg/μl)	A₂₆₀/ ₂₈₀	A₂₆₀/ ₂₃₀	Yield (μg)
1	GPT2967P	22.68	1.84	1.07	1.36
2	GPT2952P	33.98	1.92	1.57	2.04
3	NP679P	33.65	1.80	1.34	2.02
4	NP777P	39.22	1.86	1.20	2.35
5	MP795P	22.05	1.79	0.97	1.32

by NanoDrop ND-1000

DNA quality analysis by NanoDrop.

Sample ID (1) to (5) are different amniotic fluid samples. It is shown that MagCore® HF16 system can purify 1~2 μg DNA from10~15 ml amniotic fluid samples and DNA purity is with the A₂₆₀/₂₈₀ ratio of around 1.8±0.1

Genomic DNA isolations were run by gel electrophoresis on 1% agarose gel.

Lane M	RBC 1kb ladder marker
Lane 1	GPT2967P
Lane 2	GPT2952P
Lane 3	NP679P
Lane 4	NP777P
Lane 5	NP795P

1% Agarose gel electrophoresis, 110V 5 µl sample loading

Cat.No. : MCC-01

Storage And Stability : Store at Room Temperature

Packing : 36 tests / Kit

Pre-Filled Cartridge Reagent ( 36 pcs/set x 1 set)

Pipette Tip and Holder Set ( 36 pcs/packs x 1 packs)

Sample Tube ( 36 pcs/packs x 1 packs)

Elution (Eppendorf) Tube ( 36 pcs/packs x 1 packs)

PK Storage Buffer ( 1.25 ml/tube x 1 tube)

Proteinase K ( 11 mg/tube x 1 tube)

Cat.No. : MCC-02

Storage And Stability : Store at Room Temperature

Packing : 96 tests / Kit

Pre-Filled Cartridge Reagent ( 48 pcs/set x 2 set)

Pipette Tip and Holder Set ( 100 pcs/Box x 1 Box)

Sample Tube ( 100 pcs/packs x 1 packs)

Elution (Eppendorf) Tube ( 100 pcs/packs x 1 packs)

PK Storage Buffer ( 1.25 ml/tube x 2 tube)

Proteinase K ( 11 mg/tube x 2 tube)